HDAC8 Activity Assay Kit (Fluorometric) (ab156069)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Tissue Extracts
Overview
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Product name
HDAC8 Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s HDAC8 Activity Assay Kit (Fluorometric) (ab156069) detects HDAC activity in lysates.
Primarily, the HDAC8 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of HDAC inhibitors using recombinant HDAC8. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC8 activity with the HDAC8 Activity Assay Kit (Fluorometric) after immunoprecipitation with an appropriate HDAC8 specific antibody.
Applications for this kit include:
1. Monitoring the purification of HDACs including HDAC1, 2, 3 and 8 (class I).
2. Screening inhibitors or activators of HDAC8.
3. Detecting the effects of pharmacological agents on HDAC8.
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Notes
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others. -
Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests Developer 1 x 500µl Fluoro-Deacetylated Peptide (0.2 mM) 1 x 100µl Fluoro-Substrate Peptide (0.2 mM) 1 x 500µl HDAC Assay Buffer 2 x 1ml Recombinant HDAC8 1 x 500µl Stop Solution 2 x 1ml Trichostatin A (200µM) 1 x 500µl -
Research areas
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. -
Tissue specificity
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsPhosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. -
Cellular localization
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. - Information by UniProt
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Alternative names
- HD8
- HDAC 8
- Hdac8
see all -
Database links
- Entrez Gene: 55869 Human
- SwissProt: Q9BY41 Human
- Unigene: 310536 Human
Images
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ab156069 - HDAC8 Activity Assay Kit (Fluorometric)Dose dependency of recombinant HDAC8 (30min.)
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ab156069 - HDAC8 Activity Assay Kit (Fluorometric)Time course of HDAC8 reaction