HDAC1 Sumoylation Assay Kit (ab115132)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell culture extracts, Tissue Extracts
Overview
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Product name
HDAC1 Sumoylation Assay Kit -
Detection method
Colorimetric -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Quantitative -
Assay time
3h 00m -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Mammals -
Product overview
Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners.
HDAC1 is a class I histone deacetylase that plays a critical role in transcriptional repression of gene expression and has been shown to be an integral component of multiprotein co-repressor complexes. It has been observed that an important mechanism of HDAC1 activity regulation is sumoylation, which acts by potentiating HDAC1 activity.
Abcam's HDAC1 Sumoylation Assay Kit (ab115132) allows the user to measure in vivo HDAC1 sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo HDAC1 sumoylation from multiple mammalian cells/tissues extracts.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 10X Wash Buffer 1 x 25ml 1 x 50ml 8-Well Assay Strips (with Frame) 6 units 12 units 8-Well Control Strips 2 units 3 units Color Development Solution 1 x 6ml 1 x 12ml Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml Sumo Antibody, 1µg/µL 1 x 5µl 1 x 10µl Sumo Assay Buffer 1 x 2ml 1 x 4ml Sumo Protein, 1 µg/µL 1 x 6µl 1 x 12µl -
Research areas
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Alternative names
- GON 10
- HD1
- HDAC 1
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Database links
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q13547 Human
- SwissProt: O09106 Mouse
- SwissProt: Q4QQW4 Rat
- Unigene: 88556 Human
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