Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners.

HDAC1 is a class I histone deacetylase that plays a critical role in transcriptional repression of gene expression and has been shown to be an integral component of multiprotein co-repressor complexes. It has been observed that an important mechanism of HDAC1 activity regulation is sumoylation, which acts by potentiating HDAC1 activity.

Abcam's HDAC1 Sumoylation Assay Kit (ab115132) allows the user to measure in vivo HDAC1 sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo HDAC1 sumoylation from multiple mammalian cells/tissues extracts.

• Stem Cells
• Signaling Pathways
• Wnt
• HDACs

• Epigenetics and Nuclear Signaling
• Chromatin Modifying Enzymes
• Acetylation
• HDACs
• Class I

• Cardiovascular
• Heart
• Hypertrophy
• Other

• Kits/ Lysates/ Other
• Kits
• Epigenetic kits
• Sumoylation

• Epigenetics and Nuclear Signaling
• Assays & Kits
• Sumoylation