GST-Tag Protein Expression Check Kit (ab270052)
Overview
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Product name
GST-Tag Protein Expression Check Kit -
Product overview
GST-Tag Protein Expression Check Kit (ab270052) is formulated as a qualitative sandwich lateral flow assay. The conjugate pad contains a mixture of anti-GST antibody conjugated to either 40 nm Gold nanoparticles or peptide whilst the “Test line” (T line) on the nitrocellulose contains an antibody which binds the peptide. As such, a strong T line will appear in the presence of GST-tagged proteins whereas in the absence of GST-tagged protein the T line will not be visible or will appear as a faint line.
The conjugate pad of the GST-Tag Protein Expression Check strips also contains biotin-gold, which binds streptavidin on the “Control Line” (C line) of the nitrocellulose strips. Hence, the C line is assay-independent and should always appear as a strong red line; if it is not visible, the test is not valid and should be repeated.
Benefits:
Quick and easy qualitative detection of GST-tagged proteins
Compatible with cell culture media and lysate
No costly equipment required
Small sample volumes required (80 μL)
Compatible with Glutathione Affinity Resin - Amintra® (ab270237)
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Notes
This product is manufactured by Expedeon, an Abcam company. It was previously called GST-Tag Check&Go! kit. Expedeon product code 4005-0030 is the same as the 30-test size of this product.
Properties
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Storage instructions
Please refer to protocols. -
Components 30 tests ab274091 - GST-Tag Protein Expression Check Strips 1 x 30 tests ab274093 - GST-tagged protein (Lyophilized) 1 vial ab274092 - Running Buffer (10X) 2 x 1500µl
Images
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How The Kit Works
A nitrocellulose membrane carries a conjugate pad containing a mixture of anti-GST antibody conjugated to either 40 nm Gold nanoparticles or peptide and a “Test line” (T line) containing an antibody which binds the peptide. As such, a strong T line will appear in the presence of GST-tagged proteins (Figure 1-A) whereas in the absence of GST-tagged protein the T line will not be visible or will appear as a faint line (Figure 1-B). The C line is assay-independent and should always appear as a strong red line – if it is not visible, the test is not valid and should be repeated.
