Goat F(ab')2 Anti-Rat IgG - Fc (FITC), pre-adsorbed (ab6253)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - Fc (FITC), pre-adsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, Immunomicroscopy, ELISA
Overview
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Product name
Goat F(ab')2 Anti-Rat IgG - Fc (FITC), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins -
Tested applications
Suitable for: Flow Cyt, ICC/IF, Immunomicroscopy, ELISAmore details -
Minimal
cross-reactivity
Cow, Horse, Human more details -
Immunogen
Rat IgG F(c) fragment
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.8 moles FITC per mole of Goat IgG F(ab’)2 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
