Goat Anti-Mouse IgM mu chain (DyLight® 488) (ab97007)
Key features and details
- Goat Anti-Mouse IgM mu chain (DyLight® 488)
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Overview
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Product name
Goat Anti-Mouse IgM mu chain (DyLight® 488)
See all IgM secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Goat Anti-Mouse IgM mu chain (DyLight® 488) (ab97007)
Overlay histogram showing HeLa cells stained with ab130935 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130935, 1µg/1x10^6 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.
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Goat Anti-Mouse IgM mu chain (DyLight® 488) (ab97007)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.