Goat Anti-Human IgM H&L (FITC) (ab8497)
Key features and details
- Goat Anti-Human IgM H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: ICC/IF
Overview
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Product name
Goat Anti-Human IgM H&L (FITC)
See all IgM secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
Antibodies were raised in goat to human IgM -
Tested applications
Suitable for: ICC/IFmore details -
Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound. -
Conjugation notes
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
Clonality
Polyclonal -
Light chain type
unknown -
General notes
F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where IgM is depostited. -
Research areas
