Goat Anti-Human IgA H&L (FITC) (ab8500)
Key features and details
- Goat Anti-Human IgA H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: IHC-P
Overview
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Product name
Goat Anti-Human IgA H&L (FITC)
See all IgA secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
Antibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumin fractions of animal species. -
Tested applications
Suitable for: IHC-Pmore details -
Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound. -
Conjugation notes
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
Clonality
Polyclonal -
General notes
F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgG.
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Research areas