Glutathione Sepharose® (ab193267)
Overview
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Product name
Glutathione Sepharose® -
Product overview
Contents:
Supplied as a 50 % slurry in 20 % Ethanol / PBS; > 3 mg (10 µmol) glutathione per mL Sepharose® beads.
Features:
The Glutathione-S-Transferase (GST) gene fusion system has been widely used for the overexpression of foreign genes in E. Coli and other expression systems. The GST-tagged protein can be easily purified from cell lysate or media by affinity chromatography using glutathione Sepharose® beads. Abcam’s Glutathione Sepharose® is designed for the purification of GST fusion proteins and other glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of GST-fusion proteins or other glutathione-binding proteins.
Sepharose is a registered trademark of GE Healthcare
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Notes
Glutathione Sepharose® is prepared by covalently coupling glutathione to epoxy-activated 4% cross-linked Sepharose® beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity, which could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel.
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Tested applications
Suitable for: Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 10 ml 50 ml Glutathione Sepharose® 1 x 1ml 1 x 10ml 1 x 50ml -
Research areas
