Glucose Assay Kit (ab65333)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 1 µM
Overview
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Product name
Glucose Assay Kit
See all Glucose kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids -
Assay type
Quantitative -
Sensitivity
1 µM -
Range
1 µM - 10000 µM -
Assay time
0h 40m -
Product overview
Glucose Assay Kit ab65333 is a rapid, simple and sensitive assay used to quantify glucose levels in biological samples such as serum, plasma, and other body fluids, food, growth medium, etc.
In the glucose assay protocol, the glucose enzyme mix oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the amount of glucose.
The kit detects glucose in the range 1-10000 µM.
Glucose assay protocol summary:
- add samples (deproteinized) and standards to wells
- add reaction mix and incubate for 30 min at 37ºC
- analyze with microplate reader -
Notes
If you have reducing substances in your samples, we recommend using Glucose Detection Kit II (ab102517).
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Glucose Enzyme Mix (lyophilized) Green 1 vial Glucose Probe (in DMSO) Red 1 x 200µl Glucose Standard (100 nmol/µl ) Yellow 1 x 100µl -
Research areas
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Relevance
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
Images
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Functional studies- ab65333 Image from Shao W et al., PLoS One 7(1), Fig 2C. Doi: 10.1371/journal.pone.0028784. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Shao et al investigated the functional outcome of long- term curcumin supplementation on glucose homeostasis. Glucose metabolism was determined in animals with low fat diet (LFD), high fat diet (HFD) and HFD with curcumin feeding using ab65333. Intraperitoneal insulin tolerance tests(IPITT) were conducted at the end of the 26 weeks. It was concluded curcumin improves insulin sensitivity and disposal of glucose.
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Functional Studies - Glucose Assay Kit (ab65333)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Glucose Assay Kit (ab65333)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Glucose Assay Kit (ab65333)Glucose measured in cell lysates showing quantity (nmol) per million cells.
Samples with the concentration of 2x107 cells/mL were used. Samples were diluted 1.5-13.5 fold and measured colorimetrically.
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Functional Studies - Glucose Assay Kit (ab65333)Glucose measured in human biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 13.5 fold and measured colorimetrically.
