Fructose-6-Phosphate Assay Kit (Fluorometric) (ab204720)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 1 µM
Overview
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Product name
Fructose-6-Phosphate Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell Lysate, Tissue Lysate -
Sensitivity
1 µM -
Product overview
Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate (G6P) which is subsequently oxidized with the generation of a fluorescent product. This assay kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ∼ 1 µM of F6P.
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Notes
Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests F6P Assay Buffer 1 x 25ml F6P Converter (lyophilized) 1 vial F6P Enzyme Mix (lyophilized) 1 vial F6P Probe 1 x 400µl F6P Standard (10 umol, lyophilized) 1 vial F6P Substrate Mix (lyophilized) 1 vial
Images
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Fructose-6-Phosphate Assay Kit (ab204720)
Typical F6P Standard calibration curve
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Fructose-6-Phosphate Assay Kit (ab204720)Typical F6P Standard calibration curve