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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper

Fos B overexpression 293T lysate (whole cell) (ab94279)

Fos B overexpression 293T lysate (whole cell) (ab94279)
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Overview

  • Product name

    Fos B overexpression 293T lysate (whole cell)
  • General notes

    ab94279 is a 293T cell transfected lysate in which Human Fos B has been transiently over-expressed using a pCMV-Fos B plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
  • Background

    Function: FosB interacts with Jun proteins enhancing their DNA binding activity. Similarity: Belongs to the bZIP family. Fos subfamily. Contains 1 bZIP domain.

Images

  • SDS-PAGE - Fos B overexpression 293T lysate (whole cell) (ab94279)
    SDS-PAGE - Fos B overexpression 293T lysate (whole cell) (ab94279)
    ab94279 at 15µg/lane on an SDS-PAGE gel.
  • Western blot - Fos B overexpression 293T lysate (whole cell) (ab94279)
    Western blot - Fos B overexpression 293T lysate (whole cell) (ab94279)
    All lanes : Anti-Fos B antibody (ab89636) at 1/500 dilution

    Lane 1 : Fos B overexpression 293T lysate (whole cell) (ab94279)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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