Feline Galectin-3 ELISA Kit (ab273232)
Key features and details
- Sensitivity: 4.5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cat
Overview
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Product name
Feline Galectin-3 ELISA Kit
See all Galectin 3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.5 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 137.4 133% - 140% Plasma 141.3 80% - 137% Cell culture media 128.6 115% - 142% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cat -
Product overview
Feline Galectin-3 ELISA Kit (ab273232) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline Galectin-3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for feline Galectin-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-feline Galectin-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Feline Galectin-3 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Feline Galectin-3 Antibody 2 vials Feline Galectin-3 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
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Alternative names
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see all
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.