Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric) (ab273323)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell culture extracts, Tissue Homogenate
- Sensitivity: 0.5 µU
Overview
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Product name
Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell culture extracts, Tissue Homogenate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.5 µU -
Assay duration
Multiple steps standard assay -
Product overview
Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric) (ab273323) utilizes the ability of active Endothelin Converting Enzyme 1 (ECE-1) to cleave a synthetic substrate (MCA-based peptide) releasing free fluorophore, in the presence or absence of the ECE-1 Inhibitor Mix. The released fluorophore can be easily quantified using a fluorescence microplate reader. The kit uses a unique combination of substrate and inhibitor that specifically detects ECE-1 in a variety of biological samples. Contribution of other enzymes with similar catalytic properties - i.e. Endothelin Converting Enzyme 2 (ECE-2), Angiotensin-Converting Enzyme (ACE1, ACE2), and Neprilysin is compensated for within the assay.
This assay kit is simple, specific and can detect as low as 0.5 µU of ECE-1 activity.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ECE-1 (lyophilized) 1 vial ECE-1 Assay Buffer 1 x 50ml ECE-1 Inhibitor Mix (lyophilized) 1 vial ECE-1 Substrate (in DMSO) 1 x 55µl MCA Standard (5 mM) 1 x 40µl -
Research areas
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Relevance
Function: Converts big endothelin-1 to endothelin-1. Tissue specificity: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Disease: Hirschsprung disease cardiac defects and autonomic dysfunction Similarity: Belongs to the peptidase M13 family. -
Cellular localization
Cell Membrane. Single pass type II membrane protein. -
Alternative names
- ECE
- ECE 1
- ECE-1
see all
Images
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MCA Standard Curve
Example data.
MCA Standard Curve.
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Measurement of ECE-1 ActivityExample data.
Measurement of ECE-1 Activity in mouse lung extracts (1.1 µg protein), mouse heart extracts (1.5 µg protein) and HeLa lysates (1.5 µg protein). All assays were performed following kit protocol.
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ECE-1 activityExample data.
ECE-1 activity in mouse lung (1.1 µg protein) with or without Inhibitor Mix.
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Measurement of ECE-1 and ECE-2Example data.
Measurement of purified ECE-1 (5.5 ng) and ECE-2 (12 ng) activities. The kit can effectively discriminate ECE-1 activity from ECE-2 activity.