Abcam's DoubleStain IHC Kit (ab183277) is designed to use with user supplied mouse and rat primary antibody to detect two distinct antigens on mouse tissue or cell samples. This kit can be used on frozen specimens, paraffin–embedded tissues, or freshly prepared monolayer cell smears. This kit is designed not to give background with most mouse strains however there may be some, especially when using frozen tissue, that require additional blocking.

Double staining is one of most common methods used in immunohistostaining that allows detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183277) supplies the user with two polymer enzyme conjugates: Mouse HRP Polymer and Rat AP Polymer with two distinct substrates/chromogens, DAB (brown color, used with the Mouse HRP Polymer) and Permanent Red (red color, used with the Rat AP Polymer). A Primer step is used to increase specificity of antibody staining. Both enzyme conjugates are applied to the specimen at the same time and mixed on the slide. This kit offers simplified steps that make for a quicker and easier protocol than those used in a sequential procedure. Abcam's DoubleStain IHC Kit (ab183277) is non-biotin system that avoids endogenous biotin non-specific binding.

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• Multicolor chromogenic