Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
Key features and details
- Donkey Anti-Sheep IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot
Overview
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Product name
Donkey Anti-Sheep IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Sheep -
Tested applications
Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blotmore details -
Immunogen
Full length native Sheep IgG (purified).
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
IHC image of Histone H3 staining in human normal colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab128012, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6900, 1/1000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900) -
ELISA - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)ELISA results of purified ab6900 tested against purified Sheep IgG.
Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
