Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
Key features and details
- Donkey Anti-Mouse IgG H&L (DyLight® 488)
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
Overview
-
Product name
Donkey Anti-Mouse IgG H&L (DyLight® 488)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cytmore details -
Immunogen
Other Immunogen Type corresponding to Mouse IgG.
-
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875) This image is courtesy of an Abreview submitted by Charles Pallangyo.
ab7817 staining alpha smooth muscle actin in mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hour at 25°C. A DyLight® 488-conjugated donkey anti-mouse IgG H&L (ab96875) (1/1000) was used as the secondary antibody. Vimentin appears in red.
-
Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
