Diclofenac sodium salt, cyclo-oxygenase inhibitor (ab120621)
Key features and details
- Potent cyclo-oxygenase inhibitor
- CAS Number: 15307-79-6
- Purity: > 99%
- Soluble in water to 50 mM (at room temperature) and in water to 100 mM (with warming)
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Diclofenac sodium salt, cyclo-oxygenase inhibitor -
Description
Potent cyclo-oxygenase inhibitor -
Biological description
Potent non-selective COX inhibitor (IC50 values are 75 and 29 nM at COX-1 and COX-2, respectively). Potent inhibitor of phospholipase A2 in vitro and in vivo. Activates the nitric oxide-cyclic GMP-potassium channel pathway. Shows anti-inflammatory, antipyretic and analgesic effects.
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Purity
> 99% -
CAS Number
15307-79-6 -
Chemical structure
Properties
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Chemical name
2-[(2,6-Dichlorophenyl)amino]benzeneacetic acid sodium salt -
Molecular weight
318.13 -
Molecular formula
C14H10Cl2NNaO2 -
Storage instructions
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 50 mM (at room temperature) and in water to 100 mM (with warming) -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Functional Studies - Diclofenac sodium salt, cyclo-oxygenase inhibitor (ab120621)ab96732 staining Prostaglandin dehydrogenase 1 in MCF7 cells treated with diclofenac sodium salt (ab120621), by ICC/IF. Increase in Prostaglandin dehydrogenase 1 expression correlates with increased concentration of diclofenac sodium salt as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120621 (diclofenac sodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96732 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
