Daratumumab (Human) ELISA Kit (ab280327)
Key features and details
- Sensitivity: 10 ng/ml
- Range: 10 ng/ml - 1000 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Daratumumab (Human) ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10 ng/ml -
Range
10 ng/ml - 1000 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
For in vitro quantitative determination of free Daratumumab in human serum and plasma samples.
Daratumumab (Human) ELISA kit (ab280327) measures the free Daratumumab concentration in human serum or plasma with high sensitivity and reproducibility.
Standards and samples (serum or plasma) are added to the microtiter plate coated with the reactant for Daratumumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to Daratumumab captured by the reactant on the surface of the wells. Following incubation, wells are washed, and the enzymatic activity is detected by the addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Daratumumab in the sample or standard. The sample results can be determined directly using the standard curve.
Except for Daratumumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins.
Recovery rate:
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Assay Buffer 2 x 50ml Daratumumab Coated Microtiter Plate 1 unit Daratumumab Standards (S1 – S8) 8 vials HRP-conjugate Probe 1 x 12ml Plate sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml
Images
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Example Standard Curve
Typical Standard Curve. These standard curves are for demonstration only. A standard curve must be run with each assay.