D4476, CK1 inhibitor (ab120220)
Key features and details
- CK1 inhibitor
- CAS Number: 301836-43-1
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
D4476, CK1 inhibitor -
Description
CK1 inhibitor -
Biological description
Selective cell-permeable inhibitor of casein kinase 1 (CK1) . (IC50 values are 0.3 (CK1), 0.5 (ALK5), 9.1 (PKd1) and 5.8 μM (p38αMAPK)). Displays selectivity over many other protein kinases.
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Purity
> 99% -
CAS Number
301836-43-1 -
Chemical structure
Properties
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Chemical name
4-[4-(2,3-Dihydro-1,4-benzodioxin-6-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]benzamide -
Molecular weight
398.41 -
Molecular formula
C23H18N4O3 -
PubChem identifier
6419753 -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
NC(=O)c1ccc(cc1)c3nc(c2ccccn2)c(n3)c4ccc5OCCOc5c4 -
Source
Synthetic
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Research areas
Images
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Functional Studies - D4476, CK1 inhibitor (ab120220)
ab38511 staining βcatenin (phospho Thr42 + Ser45) in SK-N-SH cells treated with D4476 (ab120220), by ICC/IF. Decrease in expression of βcatenin (phospho Thr42 + Ser45) correlates with increased concentration of D4476, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120220 (D4476) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab38511 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
