D-erythro Sphingosine-1-phosphate, agonist of S1P receptors (ab141748)
Key features and details
- Endogenous signaling sphingolipid and agonist of S1P receptors
- CAS Number: 26993-30-6
- Purity: > 98%
Sparingly soluble in 1:1 ethanol:water. Addition of small amounts of acetic acid may help dissolve this product. Soluble in warm methanol.
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
D-erythro Sphingosine-1-phosphate, agonist of S1P receptors -
Description
Endogenous signaling sphingolipid and agonist of S1P receptors -
Biological description
Endogenous signaling sphingolipid and agonist of S1P receptors (EDG-GPCRs). Mobilises Ca2+ from intracellular stores via IP3 receptor independent pathways. Activates MAPK pathways. Regulates a variety of cellular responses and promotes angiogenesis. -
Purity
> 98% -
CAS Number
26993-30-6 -
Chemical structure
Properties
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Chemical name
[(E,2S,3R)-2-Amino-3-hydroxyoctadec-4-enyl] dihydrogen phosphate
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Molecular weight
379.50 -
Molecular formula
C18H38NO5P -
PubChem identifier
5283560 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Sparingly soluble in 1:1 ethanol:water. Addition of small amounts of acetic acid may help dissolve this product. Soluble in warm methanol.
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
For delivery of sphingosine-1-phosphate to cells, prepare a 125μM stock in 4mg/ml BSA as follows: Dissolve sphingosine-1-phosphate (S-1-P) in methanol (0.5 mg/ml). This may require boiling (65°C), with occasional replacement of evaporated methanol. Crushing of solid prior to addition of methanol and crushing and stirring during heating is helpful. Aliquot desired amounts of methanol stock to tubes. Evaporate the solvent with a stream of nitrogen, swirling to deposit a thin film on the inside of the tube. Aliquots may be stored at -20 °C at this point or dissolved with enough 4mg/ml BSA (fatty acid free Bovine Serum Albumin in water; 37°C, 30 min. with repeated vortexing) to make 125 µM stock.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CCCCCCCCCCCCCC=CC(C(COP(=O)(O)O)N)O -
Source
Synthetic
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Research areas
