Crystal Violet Solution (ab246820)
Overview
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Product name
Crystal Violet Solution -
General notes
Crystal Violet Solution ab246820 is the standard dye for staining gram positive bacteria.
Gram positive bacteria: Blue.
Control Tissue: Any well fixed tissue section. Air dried smear.
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Apply adequate Crystal Violet Solution to completely cover tissue section and incubate for 1-3 minute(s).
3. Rinse slide in distilled water to remove excess stain.
4. Continue with desired gram staining protocol.
Note: Gram positive bacteria that are dying, dead or being treated with antibiotics may stain variably (red).
Other products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
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Form
Liquid -
Storage instructions
Shipped at Room Temperature. Store at Room Temperature. -
Storage buffer
Constituent: 0.05% Crystal Violet -
Concentration information loading... -
Research areas
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Relevance
Gram-positive bacteria are characterized by their blue-violet colour reaction in the Gram-staining procedure. The colour reaction is caused by crystal-violet, the primary Gram-stain dye, complexing with the iodine mordant. When the decolorizer is applied, a slow dehydration of the crystal-violet/iodine complex is observed due to the closing of pores running through the cell wall. -
Cellular localization
Whole cell -
Alternative names
- Gram +ve bacteria
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Cellular localization
Whole cell
