Complex II WB Antibody Cocktail (ab110410)
Key features and details
- Assay type: Quantitative
Overview
-
Product name
Complex II WB Antibody Cocktail
See all Complex II kits -
Assay type
Quantitative -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Product overview
Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).
Cocktail Antibodies:
Mouse C-V-alpha [15H4C4] monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse C-II-30 (FeS) [21A11AE7] monoclonal:
Amount: 250 µg
Working concentration: 5 µg/ml
Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:
Amount: 5 µg
Working concentration: 0.1 µg/ml
-
Notes
Related products
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
-
Tested applications
Suitable for: WBmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 305 µg Pre-mixed solution of 3 monoclonal antibodies 1 x 305µg -
Research areas
-
Relevance
Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma. -
Alternative names
- SDH
- Succinate coenzyme Q reductase
- Succinate dehydrogenase
-
Database links
- Entrez Gene: 6390 Human
- Entrez Gene: 6389 Human
- Entrez Gene: 6391 Human
- SwissProt: P31040 Human
- SwissProt: P21912 Human
- SwissProt: Q99643 Human
- SwissProt: O14521 Human
- SwissProt: 6392 Human
see all
Images
-
Western blot - Complex II WB Antibody Cocktail (ab110410)All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilution
Lane 1 : human heart mitochondria at 10 µg
Lane 2 : Immunoprecipitated Complex II at 0.5 µg
The Western blot cocktail ab110410 contains two Complex II subunits SDHA (70 kDa) and SDHB (30 kDa)and a general mitochondrial marker ATP synthase alpha subunit (F1α, 55 kDa). All three are present in the mitochondrial sample (1). The Complex II sample purified by Complex II immunoprecipitation beads (ab109799) shows only Complex II subunits (2).
