Citrulline Assay Kit (Fluorometric) (ab273309)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Food samples, Other biological fluids, Serum
- Sensitivity: 2 µM
Overview
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Product name
Citrulline Assay Kit (Fluorometric)
See all Citrulline kits -
Detection method
Fluorescent -
Sample type
Serum, Other biological fluids, Food samples -
Assay type
Quantitative -
Sensitivity
2 µM -
Assay duration
Multiple steps standard assay -
Product overview
Citrulline Assay Kit (Fluorometric) (ab273309) provides a rapid, specific, and easy method for the measurement of total Citrulline concentrations in a wide variety of samples.
In this enzymatic assay, Citrulline is converted into a series of intermediates, which further reacts with a probe producing a stable fluorescent signal (Ex/Em = 535/587 nm). The kit is simple, easy to perform, sensitive and is high throughput adaptable.
It can detect as low as 2 µM Citrulline in biological samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Othermore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Citrulline Assay Buffer 1 x 25ml Citrulline Buffer Supplement (Lyophilized) 1 vial Citrulline Cofactor Mix 1 x 200µl Citrulline Converter Mix (Lyophilized) 1 vial Citrulline Developer Mix 1 x 200µl Citrulline Enzyme Mix (Lyophilized) 1 vial Citrulline Probe 1 x 200µl Citrulline Standard (Lyophilized) 1 vial -
Research areas
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Relevance
The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.
Images
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Citrulline Standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Citrulline Standard Curve.
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Specificity of the detection of CitrullineSpecificity of the detection of Citrulline over other amino acids. Other amino acids were tested at a 10-fold molar excess (each AA: 10 nmol) vs Citrulline (1 nmol).
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Estimations of Citrulline in different samplesEstimations of Citrulline in 2 human serum samples (10 and 40 µl in each well respectively) and watermelon juice (4 µl of 100X dilution). Citrulline concentrations were 33.50 µM and 9.11 µM in human serum respectively and 10.81 mM in watermelon juice.