Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm)
See all Cell Migration/Chemotaxis kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Product overview
Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693) utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. The assay is easy to use, sensitive and adaptable to high-throughput systems.
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Notes
Cell migration is a process by which cells move from one location to another. Cell motility is observed in unicellular organisms, and is essential for the development and maintenance of multicellular organisms. Cells often migrate in response to specific external stimuli, including chemical & mechanical signals. Errors during this process have serious consequences, including intellectual disability, vascular disease, tumor formation and metastasis.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cell Dissociation Solution 1 x 10ml Cell Dye 1 x 1.5ml Cell Migration Chamber 1 unit Control Migration Inducer 1 x 300µl Wash Buffer 1 x 50ml
Images
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Cell Migration.
Monocytes/macrophage cells were starved overnight and treated with Control Migration Inducer for 24 h, 48 h or left untreated (Cnt: control cells). Treatment with Control Migration Inducer demonstrated a significant increase in migration with time as compared to control cells.
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Standard Curve.Monocytes/macrophage cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Dye and RFU (Ex/Em = 530/590 nm) was measured.
