Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm)
See all Cell Migration/Chemotaxis kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mouse, Human -
Product overview
Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) (ab235696) utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.
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Notes
Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical and mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 12 tests Cell Dissociation Solution 1 x 6ml Cell Dye 1 x 1.5ml Cell Migration Chamber 1 unit Control Migration Inducer 1 x 300µl Wash Buffer 1 x 25ml
Images
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Standard Curve.
Monocytes/macrophage cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol.
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Cell InvasionMonocytes/macrophage cells were starved overnight and treated with Control (Cnt) Invasion Inducer for 24 or 48 hours or left untreated. Treatment with Control Invasion Inducer demonstrated a significant increase in invasion with time. Control reading was subtracted from inducer reading.
