Cantharidin, Protein phosphatases 1 and 2A (PP1, PP2A) inhibitor (ab120967)
Key features and details
- Protein phosphatases 1 and 2A (PP1, PP2A) inhibitor
- CAS Number: 56-25-7
- Soluble in DMSO to 100 mM
- Form / State: Solid
Overview
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Product name
Cantharidin, Protein phosphatases 1 and 2A (PP1, PP2A) inhibitor -
Description
Protein phosphatases 1 and 2A (PP1, PP2A) inhibitor -
Biological description
Protein phosphatases 1 and 2A (PP1, PP2A) inhibitor (Ki values are 1.1 and 0.19 μM, respectively). Toxin found in Mylabris phalerata. Possesses various biological activities including anticancer, antitumor activity.
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CAS Number
56-25-7 -
Chemical structure
Properties
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Chemical name
rel-(3aR,4S,7R,7aS)-Hexahydro-3a,7a-dimethyl-4,7-epoxyisobenzofuran-1,3-dione -
Molecular weight
196.20 -
Molecular formula
C10H12O4 -
PubChem identifier
5944 -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC12C3CCC(C1(C(=O)OC2=O)C)O3 -
Research areas
Images
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Functional Studies - Cantharidin, Protein phosphatases 1 and 2A (PP1, PP2A) inhibitor (ab120967)ab7960 staining p21 in PANC-1 cells treated with cantharidin (ab120967), by ICC/IF. Increase of p21 expression correlates with increased concentration of cantharidin, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120967 (cantharidin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7960 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.