Canine IFN-alpha ELISA Kit (ab273185)
Key features and details
- Sensitivity: 0.18 ng/ml
- Range: 0.164 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine IFN-alpha ELISA Kit
See all IFN alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.18 ng/ml -
Range
0.164 ng/ml - 40 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 100.9 91% - 109% Plasma 98.4 91% - 106% Cell culture media 95.38 84% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine IFN-alpha ELISA Kit (ab273185) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IFN-alpha in serum (Canine IFN-alpha concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA as an anti-coagulant. Citrate and heparin are not recommended as anti-coagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Canine IFN-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IFN-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IFN-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IFN-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Canine IFN-alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine IFN-alpha Antibody 2 vials Canine IFN-alpha Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
A member of the type 1 IFN family of related proteins produced by leucocytes and fibroblasts. IFN alpha 1 has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
Cellular localization
Secreted -
Alternative names
- IFL
- IFN
- IFN alpha
see all
Images
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Canine IFN-alpha ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
