Canine Galectin-3 ELISA Kit (ab273178)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2.05 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
-
Product name
Canine Galectin-3 ELISA Kit
See all Galectin 3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
2.05 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 118.2 90% - 145% Plasma 115.6 90% - 148% Cell culture media 113 75% - 147% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine Galectin-3 ELISA Kit (ab273178) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Galectin-3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine Galectin-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine Galectin-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine Galectin-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine Galectin-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Canine Galectin-3 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine Galectin-3 Antibody 2 vials Canine Galectin-3 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
-
Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
-
Alternative names
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see all -
Database links
Images
-
Canine Galectin-3 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.