Recombinant human Galectin 3 protein (ab78583)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
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Product name
Recombinant human Galectin 3 protein
See all Galectin 3 proteins and peptides -
Biological activity
The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL. Corresponding to a specific activity of 5 x 102 units/mg.
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Purity
> 95 % SDS-PAGE.
Purity Approximately 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. Endotoxin level as measured by LAL is -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI -
Predicted molecular weight
26 kDa -
Amino acids
1 to 250
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.164% Sodium phosphate, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile water at 0.1-1.0 mg/ml, which can then be further diluted to other aqueous solutions.
Images
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Western blot - Recombinant human Galectin 3 protein (ab78583)ab78583 used in Western Blot. Figure: 1 ug under (+) reducing and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human Galectin-3 has a predicted MW of 26 kDa.
