Canine CKM ELISA Kit (ab197749)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 20 pg/ml
- Range: 0.08 ng/ml - 5 ng/ml
- Sample type: Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine CKM ELISA Kit
See all Creatine Kinase MM kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 7.88% Inter-assay Sample n Mean SD CV% Serum 3 7.18% -
Sample type
Serum, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
20 pg/ml -
Range
0.08 ng/ml - 5 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102.61 102.54% - 102.67% 1X Cell Extraction Buffer PTR 101.35 96.12% - 111.33% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Dog
Does not react with: Mouse, Hamster -
Product overview
Canine CKM ELISA Kit (ab197749) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CKM protein in serum and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Canine CKM with 20 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Creatine kinase (CK) catalyzes creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and producing adenosine diphosphate (ADP). This process is reversible, thus PCr and ADP can be used to generate ATP and creatine. There are three known cytoplasmic isoenzymes (CKMM- skeletal muscle, CKMB-myocardium, and CKBB- nervous tissue). Phosphocreatine serves as an energy storage for rapid regeneration of ATP. Creatine is used in tissues with high energy demands, such as skeletal muscle, heart, and brain.
Expression of CKM in serum are based on muscle mass, age, gender, race, and physical activity. Skeletal muscle expresses approximately 98% CKMM and 1% CKMB, myocardium expresses 70% CKMM and 25-30% CKMB, and neural tissue expresses a majority of CKBB.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 10X CKM Capture Antibody 1 x 600µl 10X CKM Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Canine CKM Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Cytoplasmic -
Alternative names
- CKM
- CKMM
- creatine kinase M chain
see all -
Database links
- Entrez Gene: 476435 Dog
- SwissProt: P05123 Dog
Images
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Other - Canine CKM ELISA Kit (ab197749)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of CKM standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of CKM standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CKM in canine serum samples.The concentrations of CKM were measured in duplicates, interpolated from the CKM standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 603.95 ng/mL in serum.