cAMP Phosphodiesterase Activity Assay Kit (Fluorometric) (ab273342)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Purified protein, Suspension cells, Tissue
Overview
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Product name
cAMP Phosphodiesterase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells, Purified protein -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
cAMP Phosphodiesterase Activity Assay Kit (Fluorometric) (ab273342) provides a rapid, sensitive and straightforward way to measure cAMP Phosphodiesterase (cPDE) activity in various sample types. In this assay, AMP produced by cPDE activity is metabolized by the enzyme mix, developer mix and converter mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured at Ex/Em = 535/587 nm.
This assay can detect cPDE activity as low as 0.1 µU per well.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AMP Standard 1 x 100µl cPDE Assay Buffer 1 x 25ml cPDE Converter Lyophilized 1 vial cPDE Developer Lyophilized 1 vial cPDE Enzyme Mix 1 x 200µl cPDE Positive Control Lyophilized 1 vial cPDE Probe 1 x 200µl cPDE Substrate Lyophilized 1 vial
Images
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Example data
Example data.
AMP Standard Curve.
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Example dataExample data.
Reaction kinetics of cPDE Positive Control.
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Example dataExample data.
cPDE activity in Rat lung and brain lysate. For activity determination, experiments were run in duplicates, with 0.9-3.6 µg protein (lung) or 0.1-0.4 µg protein (brain) loaded per well.
