Astana Biomed Group, an authorized Abcam distributor in Central Asia

cAMP Phosphodiesterase Activity Assay Kit (Fluorometric) (ab273342)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Fluorescent
  • Platform: Microplate
  • Sample type: Adherent cells, Purified protein, Suspension cells, Tissue

Overview

  • Product name

    cAMP Phosphodiesterase Activity Assay Kit (Fluorometric)

  • Detection method

    Fluorescent

  • Sample type

    Tissue, Adherent cells, Suspension cells, Purified protein

  • Assay type

    Enzyme activity (quantitative)

  • Assay duration

    Multiple steps standard assay

  • Product overview

    cAMP Phosphodiesterase Activity Assay Kit (Fluorometric) (ab273342) provides a rapid, sensitive and straightforward way to measure cAMP Phosphodiesterase (cPDE) activity in various sample types. In this assay, AMP produced by cPDE activity is metabolized by the enzyme mix, developer mix and converter mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured at Ex/Em = 535/587 nm.


    This assay can detect cPDE activity as low as 0.1 µU per well.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    AMP Standard 1 x 100µl
    cPDE Assay Buffer 1 x 25ml
    cPDE Converter Lyophilized 1 vial
    cPDE Developer Lyophilized 1 vial
    cPDE Enzyme Mix 1 x 200µl
    cPDE Positive Control Lyophilized 1 vial
    cPDE Probe 1 x 200µl
    cPDE Substrate Lyophilized 1 vial

Images

  • Example data
    Example data

    Example data.

    AMP Standard Curve.

  • Example data
    Example data

    Example data.

    Reaction kinetics of cPDE Positive Control.

  • Example data
    Example data

    Example data.

    cPDE activity in Rat lung and brain lysate. For activity determination, experiments were run in duplicates, with 0.9-3.6 µg protein (lung) or 0.1-0.4 µg protein (brain) loaded per well.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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