Bovine IL-2 ELISA Kit (ab193682)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.2 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Overview
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Product name
Bovine IL-2 ELISA Kit
See all IL-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.2 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 133.7 126% - 142% Serum 194.8 125% - 145% Plasma 102.1 90% - 111% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cow -
Product overview
Abcam's IL-2 Bovine ELISA Kit (ab193682) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine IL-2 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for bovine IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-2 present in the samples. The wells are washed and biotinylated anti-bovine IL-2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated Bovine IL-2 Detection Antibody 2 vials Bovine IL-2 Standards (lyophilized) 2 vials Pre-coated bovine IL-2 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all
Images
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Sandwich ELISA -ab193682 Bo IL-2
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Sandwich ELISA - ab193682 Bovine IL-2