Biotin Anti-TNF alpha antibody (ab271247)
Key features and details
- Biotin Goat polyclonal to TNF alpha
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-TNF alpha antibody
See all TNF alpha primary antibodies -
Description
Biotin Goat polyclonal to TNF alpha -
Host species
Goat -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB Recombinant fragment
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Immunogen
Recombinant fragment corresponding to Human TNF alpha aa 50 to the C-terminus.
Database link: P01375
Properties
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Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-TNF alpha antibody (ab271247)
Western blot analysis of varying levels of Recombinant human TNF-α protein using ab271247 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
ELISA - Biotin Anti-TNF alpha antibody (ab271247)Human TNF alpha was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271247. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant TNF alpha protein.
