Biotin Anti-Stem Cell Factor antibody (ab271223)
Key features and details
- Biotin Rabbit polyclonal to Stem Cell Factor
- Suitable for: WB, Sandwich ELISA
- Reacts with: Rat, Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Stem Cell Factor antibody
See all Stem Cell Factor primary antibodies -
Description
Biotin Rabbit polyclonal to Stem Cell Factor -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA RatWB Recombinant fragment
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Immunogen
Recombinant fragment corresponding to Rat Stem Cell Factor aa 1-200.
Database link: P21581
Properties
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Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-Stem Cell Factor antibody (ab271223)
To detect rat Stem Cell Factor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant rat Stem Cell Factor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant rat SCF, respectively.
Non-reducing conditions.
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Sandwich ELISA - Biotin Anti-Stem Cell Factor antibody (ab271223)Rat Stem Cell Factor was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271223.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant rat Stem Cell Factor protein.