Betulinic acid, TGR5 agonist (ab120654)
Key features and details
- Anticancer agent. TGR5 agonist.
- CAS Number: 472-15-1
- Soluble in DMSO to 50 mM (with warming)
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Betulinic acid, TGR5 agonist -
Description
Anticancer agent. TGR5 agonist. -
Biological description
Triterpenoid with various biological activities. Apoptosis inducer, with anticancer properties. Also shows anti-HIV, antimalarial and anti-inflammatory activity. TGR5 agonist (EC50 = 1.04 μM).
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CAS Number
472-15-1 -
Chemical structure
Images
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Functional Studies - Betulinic acid, TGR5 agonist (ab120654)
ab32557 staining p38 (phospho T180 + Y182) in MALME-3M cells treated with betulinic acid (ab120654), by ICC/IF. Increase in expression of p38 (phospho T180 + Y182) correlates with increased concentration of betulinic acid, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120654 (betulinic acid ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32557 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.