BAP29 overexpression 293T lysate (whole cell) (ab94232)
Overview
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Product name
BAP29 overexpression 293T lysate (whole cell) -
General notes
ab94232 is a 293T cell transfected lysate in which Human BAP29 has been transiently over-expressed using a pCMV-BAP29 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
BAP29 and the related protein BAP31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Both BAP29 and BAP31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors. Binding of the BAP29/BAP31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that BAP29 and BAP31 may act as chaperones for transmembrane regions of various proteins. There are multiple isoforms of BAP29.
Images
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SDS-PAGE - BAP29 overexpression 293T lysate (whole cell) (ab94232)ab94232 at 15µg/lane on an SDS-PAGE gel.
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Western blot - BAP29 overexpression 293T lysate (whole cell) (ab94232)All lanes : Anti-BAP29 antibody (ab57493) at 1/500 dilution
Lane 1 :BAP29 overexpression 293T lysate (whole cell) (ab94232)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution