ATPase Assay Kit (Colorimetric) (ab234055)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
Overview
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Product name
ATPase Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Range
1 nmol/well - 5 nmol/well -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ATPase Assay Kit (Colorimetric) (ab234055) provides a quick and easy method for monitoring ATPase activity in cell and tissue lysates.
In the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, in which phosphate reacts with malachite green, a strong, stable chromophore is generated (OD 650 nm).
The assay is simple, sensitive, high-throughput adaptable and can detect ATPase Activity less than 0.005 U/L.
ATPases catalyzes the decomposition of ATP into ADP and a free phosphate ion. This assay can be used in the study of the many classes of ATPases including Na+ /K+ -ATPase, H + /K+ -ATPase, Ca2+ -ATPase, etc.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests ATPase Assay Buffer 1 x 25ml ATPase Developer 1 x 3ml ATPase Positive Control 1 vial ATPase Substrate 2 vials Phosphate Standard (10 mM) 1 x 0.5ml
Images
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Phosphate Standard Curve.
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Specific ATPase Activity were calculated in lysates prepared from Rat Heart (35 µg), Rat Kidney (15 µg), and Hela Cell Lysate (5.4 µg).