Ascorbic Acid Assay Kit (ab65346)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Tissue Extracts, Urine
Overview
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Product name
Ascorbic Acid Assay Kit
See all Ascorbic acid kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Range
0.01 nmol/well - 10 nmol/well -
Assay time
0h 30m -
Product overview
Ascorbic Acid Assay Kit ab65346 provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples.
In the ascorbic acid assay protocol, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.
The assay can detect 0.01-10 nmol of ascorbic acid per assay.
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Notes
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Ascorbic Acid Assay Buffer WM 1 x 25ml Ascorbic Acid Enzyme Mix (lyophilized) Green 1 vial Ascorbic Acid Probe (DMSO) Red 1 x 0.2ml Ascorbic Acid Standard (20 µmole) Yellow 1 vial Catalyst Blue 1 x 500µl -
Research areas
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Relevance
Ascorbic Acid (C6H8O6) is an essential nutrient in human diets, and is necessary to maintain connective tissue and bone. The biologically active form of ascorbic acid is vitamic C, a water soluble vitamin which is the L-enantiomer form of ascorbic acid. Humans have lost the ability to synthesize ascorbic acid and must obtain in the diet. Ascorbic acid is an electron donor for enzymes involved in collagen hydroxylation, biosynthesis of carnitine and norepinephrine, tyrosine metabolism and amidatin of peptide hormones. The ability of vitamin C to donate electrons also makes it a potent water-soluble anti-oxidant that readily scavenges free radicals. -
Alternative names
- Vitamin C
Images
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Functional studies - Ascorbic Acid Assay Kit (ab65346)
Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtracted
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Functional Studies - Ascorbic Acid Assay Kit (ab65346)Ascorbic Acid Standard Curve.
