Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require EDTA buffer (pH=8.0) pretreatment.

This product contains detergent for emulsification of the paraffin.

1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.

PROTOCOL:

1. Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
2. Wash sections with hot tap water for 1 minute.
3. Wash sections in buffer for 2x3 minutes.
4. Continue with IHC protocol as normal.

Other kits and reagents for IHC

Other antigen retrieval buffers include:

Citrate buffer pH 6.0 ab93678, Tris-EDTA buffer pH 9.0 ab93684, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.