10x EDTA Buffer pH 8.0 (ab64216)
Overview
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Product name
10x EDTA Buffer pH 8.0 -
Tested applications
Suitable for: IHC-Pmore details -
General notes
EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).
Recommended Procedure: Microwave
- Deparaffinize and rehydrate tissue sections.
- Dilute the product 1:10 with deionized water.
- Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
- Keep the slides warm by heating for 10min at LOW power.
- Let coplin jar sit in microwave for at least 20min.
- Remove and rinse with buffer.
- Proceed with staining.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
pH: 7.7
Constituent: 0.29% EDTA
Buffer 10x concentrated. -
Concentration information loading... -
Research areas
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Relevance
EDTA buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies. -
Alternative names
- Ethylenediaminetetraacetic acid buffer
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 10x EDTA Buffer pH 8.0 (ab64216)ab64216 Human Melanoma stained with an anti-S-100 antibody diluted with Antibody Diluent (ab64211)