Chemiluminescent HRP Substrate (membrane) (ab5801)
Overview
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Product name
Chemiluminescent HRP Substrate (membrane) -
Product overview
A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.
The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities
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Notes
Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.
Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.
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Tested applications
Suitable for: WBmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 kit luminol reagent. 1 x 20ml reaction buffer. 2 x 20ml -
Research areas
