Anti-ZSCAN4 antibody (ab97748)
Key features and details
- Rabbit polyclonal to ZSCAN4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZSCAN4 antibody
See all ZSCAN4 primary antibodies -
Description
Rabbit polyclonal to ZSCAN4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Human kidney tissue lysate as well as the following whole cell lysates: HEK293; A498; HuES7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications GorillaOrangutanApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).Target
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Function
Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells. -
Sequence similarities
Contains 4 C2H2-type zinc fingers.
Contains 1 SCAN box domain. -
Cellular localization
Nucleus. Chromosome > telomere. - Information by UniProt
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Database links
- Entrez Gene: 201516 Human
- Omim: 613419 Human
- SwissProt: Q8NAM6 Human
- SwissProt: A2T7G6 Orangutan
- Unigene: 469663 Human
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Alternative names
- FLJ35105 antibody
- MGC126787 antibody
- MGC126789 antibody
see all
Images
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Western blot - Anti-ZSCAN4 antibody (ab97748)All lanes : Anti-ZSCAN4 antibody (ab97748) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 4 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The predicted molecular weight of ZSCAN4 is 49 kDa (SwissProt), however we expect to observe a banding pattern at 58 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (0)
ab97748 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ZSCAN4 antibody (ab97748)All lanes : Anti-ZSCAN4 antibody (ab97748) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 4 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The predicted molecular weight of ZSCAN4 is 49 kDa (SwissProt), however we expect to observe a banding pattern at 58 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
