Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - BSA and Azide free (ab250557)
![Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - BSA and Azide free (ab250557)](https://www.abcam.com/ps/products/250/ab250557/Images/ab250557-402996-anti-zonula-occludens-protein-3-zo3-antibody-epr13414-bsa-and-azide-free-western-blot.jpg "Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - BSA and Azide free (ab250557)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13414] to Zonula occludens protein 3/ZO3 - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - BSA and Azide free
See all Zonula occludens protein 3/ZO3 primary antibodies -
Description
Rabbit monoclonal [EPR13414] to Zonula occludens protein 3/ZO3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250557 is the carrier-free version of ab181991 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250557 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Zonula occludens protein 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13414 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - BSA and Azide free (ab250557)All lanes : Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991) at 1/100000 dilution
Lane 1 : Caco2 cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 103 kDaThis data was developed using ab181991, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - BSA and Azide free (ab250557)](https://www.abcam.com/ps/products/250/ab250557/Images/ab250557-1-anti-zonula-occludens-protein-3-zo3-antibody-epr13414-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - BSA and Azide free (ab250557)This data was developed using ab181991, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cell line staining Zonula occludens protein 3 using ab181991 at a 1/250 dilution (green). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200. Counter stain: Dapi (blue). -
![Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - BSA and Azide free (ab250557)](https://www.abcam.com/ps/products/250/ab250557/Images/ab250557-3-benefits-of-recombinant-antibodies.png)
Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - BSA and Azide free (ab250557)