Anti-ZNHIT1 antibody [EPR20922-47] - BSA and Azide free (ab275036)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20922-47] to ZNHIT1 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ZNHIT1 antibody [EPR20922-47] - BSA and Azide free
See all ZNHIT1 primary antibodies -
Description
Rabbit monoclonal [EPR20922-47] to ZNHIT1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, MEF, C2C12, HEK-293T and PC12 whole cell lysates; Mouse E13.5, E15.5 and E18.5 heart tissue lysates. IP: HeLa whole cell lysate.
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General notes
ab275036 is the carrier-free version of ab238125. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20922-47 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ZNHIT1 antibody [EPR20922-47] (ab238125) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
Lane 3 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 4 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaThis data was developed using ab238125, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 70 seconds.
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This data was developed using ab238125, the same antibody clone in a different buffer formulation.
ZNHIT1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate with ab238125 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238125 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 ug
Lane 2: ab238125 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238125 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 76 seconds.
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All lanes : Anti-ZNHIT1 antibody [EPR20922-47] (ab238125) at 1/1000 dilution
Lane 1 : Wild-type mouse E13.5 heart tissue lysate
Lane 2 : ZNHIT1 conditional knockout mouse E13.5 heart tissue lysate
Lane 3 : Wild-type mouse E15.5 heart tissue lysate
Lane 4 : ZNHIT1 conditional knockout mouse E15.5 heart tissue lysate
Lane 5 : Wild-type mouse E18.5 heart tissue lysate
Lane 6 : ZNHIT1 conditional knockout mouse E18.5 heart tissue lysate
Lysates/proteins at 20 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaThis data was developed using ab238125, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Lysates were kindly provided by an anonymous collaborator.
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