Anti-ZNF316 antibody (ab176708)
Key features and details
- Rabbit polyclonal to ZNF316
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNF316 antibody -
Description
Rabbit polyclonal to ZNF316 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ZNF316 aa 475-525. The exact sequence is proprietary.
Sequence:HQAVHTADRPHCCPDCGQAFRLRADFQRHRRGGGCAEAGGDGPRREPGET A
Database link: A6NFI3 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176708 was affinity purified using an epitope specific to ZNF316 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ZNF316 antibody (ab176708)All lanes : Anti-ZNF316 antibody (ab176708) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-ZNF316 antibody (ab176708)ab176708 at 0.4 µg/ml detecting ZNF316 in HeLa whole cell lysate by WB following IP.
Lane 1: ab176708 at 6 µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of ZNF316.
Lane 3: Control IgG.In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds.