Anti-ZKSCAN3 antibody (ab223477)
Key features and details
- Rabbit polyclonal to ZKSCAN3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZKSCAN3 antibody
See all ZKSCAN3 primary antibodies -
Description
Rabbit polyclonal to ZKSCAN3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human ZKSCAN3 aa 8-275.
Database link: Q9BRR0 -
Positive control
- WB: Mouse heart, spleen and lung lysates. IHC-P: Human lymph node tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ZKSCAN3 antibody (ab223477)All lanes : Anti-ZKSCAN3 antibody (ab223477) at 1/500 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse lung lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44,61 kDa
Observed band size: 44,61 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-ZKSCAN3 antibody (ab223477)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZKSCAN3 (green) using ab223477 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZKSCAN3 antibody (ab223477)Paraffin-embedded human lymph node tissue stained for ZKSCAN3 using ab223477 at 1/100 dilution in immunohistochemical analysis.
