Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab65767)
Key features and details
- Rabbit polyclonal to Zinc finger MIZ domain-containing protein 1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Zinc finger MIZ domain-containing protein 1 antibody
See all Zinc finger MIZ domain-containing protein 1 primary antibodies -
Description
Rabbit polyclonal to Zinc finger MIZ domain-containing protein 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Zinc finger MIZ domain-containing protein 1 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90597) -
Positive control
- This antibody gave a positive signal in human testis tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
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Function
Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation. -
Tissue specificity
Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes. -
Sequence similarities
Contains 1 SP-RING-type zinc finger. -
Domain
The C-terminal proline-rich domain possesses a significant intrinsic transcriptional activity. This activity is inhibited by the N-terminus in the full-length protein. -
Cellular localization
Nucleus speckle. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 57178 Human
- Omim: 607159 Human
- SwissProt: Q9ULJ6 Human
- Unigene: 193118 Human
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Alternative names
- hZIMP10 antibody
- KIAA1224 antibody
- MIZ antibody
see all
Images
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Western blot - Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab65767)All lanes : Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab65767) at 1 µg/ml
Lane 1 : Human Ovary Tissue Lysate
Lane 2 : Human Prostate Tissue Lysate
Lane 3 : Human Spleen Tissue Lysate
Lane 4 : Human Testis Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 115 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab65767 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab65767)ICC/IF image of ab65767 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65767, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
Protocols
Datasheets and documents
References (0)
ab65767 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab65767)All lanes : Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab65767) at 1 µg/ml
Lane 1 : Human Ovary Tissue Lysate
Lane 2 : Human Prostate Tissue Lysate
Lane 3 : Human Spleen Tissue Lysate
Lane 4 : Human Testis Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 115 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab65767 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab65767)ICC/IF image of ab65767 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65767, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
