Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZHX2 antibody [EPR17303] (ab205532)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17303] to ZHX2
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ZHX2 antibody [EPR17303]
    See all ZHX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17303] to ZHX2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293 and NIH/3T3 whole cell lysates; human fetal kidney lysate; rat brain and heart lysates; HeLa nuclear fraction. ICC/IF: HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17303

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    All lanes : Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    All lanes : Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    All lanes : Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

    Lane 1 : HeLa cytosolic fraction
    Lane 2 : HeLa nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)
    Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution + NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody [EPR17303] (ab205532)
    Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody [EPR17303] (ab205532)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ZHX2 with ab205532 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:-
    -ve control 1: - ab205532 at 1/250 dilution, followed by  Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: - Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-ZHX2 antibody [EPR17303] (ab205532)
    Flow Cytometry - Anti-ZHX2 antibody [EPR17303] (ab205532)

    Flow Cytometry analysis of HeLa cells labelling ZHX2 with ab205532 at 1/80 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Anti-ZHX2 antibody [EPR17303] (ab205532)
    Anti-ZHX2 antibody [EPR17303] (ab205532)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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