Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 243 ₸

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Anti-ZHX2 antibody [EPR17303] (ab205532)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17303] to ZHX2
Suitable for: ICC/IF, WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-ZHX2 antibody [EPR17303]
See all ZHX2 primary antibodies
Description

Rabbit monoclonal [EPR17303] to ZHX2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HEK-293 and NIH/3T3 whole cell lysates; human fetal kidney lysate; rat brain and heart lysates; HeLa nuclear fraction. ICC/IF: HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17303
Isotype

IgG
Research areas

Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)

All lanes : Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 92 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)

Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution + Human fetal kidney lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 92 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)

All lanes : Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 92 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)

All lanes : Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

Lane 1 : HeLa cytosolic fraction
Lane 2 : HeLa nuclear fraction

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 92 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532)

Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution + NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 92 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody [EPR17303] (ab205532)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ZHX2 with ab205532 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: - ab205532 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: - Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-ZHX2 antibody [EPR17303] (ab205532)

Flow Cytometry analysis of HeLa cells labelling ZHX2 with ab205532 at 1/80 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Anti-ZHX2 antibody [EPR17303] (ab205532)

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