Anti-YTHDC1 antibody (ab122340)
Key features and details
- Rabbit polyclonal to YTHDC1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-YTHDC1 antibody
See all YTHDC1 primary antibodies -
Description
Rabbit polyclonal to YTHDC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to internal sequence amino acids 69-160 (LVSKPLSSSV SNNKRIVSTK GKSATEYKNE EYQRSERNKR LDADRKIRLS SSASREPYKN QPEKTCVRKR DPERRAKSPT PDGSERIGLE VD) of Human YTHDC1
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Positive control
- IHC-P: Human esophagus, fallopian tube, duodenum, endometrium and testis tissues. WB: RT-4 and U-251 MG cell lysates. ICC/IF: HeLa and U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-YTHDC1 antibody (ab122340)All lanes : Anti-YTHDC1 antibody (ab122340) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 85 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody (ab122340)Paraffin-embedded human testis tissue stained for YTHDC1 using ab122340 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody (ab122340)Paraffin-embedded human endometrium tissue stained for YTHDC1 using ab122340 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody (ab122340)Paraffin-embedded human duodenum tissue stained for YTHDC1 using ab122340 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-YTHDC1 antibody (ab122340)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for YTHDC1 (green) using ab122340 at 4 µg/ml in ICC/IF.
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Immunocytochemistry/ Immunofluorescence - Anti-YTHDC1 antibody (ab122340)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab122340 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody (ab122340)ab122340, at a 1/400 dilution, staining YTHDC1 in paraffin-embedded Human esophagus tissue by immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody (ab122340)Immunohistochemical staining of Human fallopian tube shows strong nuclear positivity in glandular cells using ab122340
