Anti-XPNPEP1 antibody [OTI1E3] (ab123929)
![Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.com/ps/products/123/ab123929/Images/XPNPEP1-Primary-antibodies-ab123929-1.jpg "Anti-XPNPEP1 antibody [OTI1E3] (ab123929)")
Key features and details
- Mouse monoclonal [OTI1E3] to XPNPEP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-XPNPEP1 antibody [OTI1E3]
See all XPNPEP1 primary antibodies -
Description
Mouse monoclonal [OTI1E3] to XPNPEP1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human XPNPEP1. Produced in HEK293T cells (NP_065116).
Database link: Q9NQW7 -
Positive control
- WB: HEK-293T cells transfected with pCMV6-ENTRY XPNPEP1 cDNA; HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC12 and MCF7 whole cell lysate. IHC-P: Human thyroid and prostate tissues. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab123929 is purified from Mouse ascites fluid by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI1E3 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY XPNPEP1, stained for XPNPEP1 (Green) using ab123929 at 1/100 dilution in ICC/IF.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.com/ps/products/123/ab123929/Images/XPNPEP1-Primary-antibodies-ab123929-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)Paraffin-embedded human thyroid tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.com/ps/products/123/ab123929/Images/XPNPEP1-Primary-antibodies-ab123929-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)Paraffin-embedded human prostate tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.
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![Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.com/ps/products/123/ab123929/Images/XPNPEP1-Primary-antibodies-ab123929-5.jpg)
Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)All lanes : Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY XPNPEP1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 70 kDa
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![Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.com/ps/products/123/ab123929/Images/ab123929-255898-pic1.jpg)
Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)All lanes : Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/200 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SVT2
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 70 kDa
